Bioengineering is a field of study that deals with the application of engineering practices to biology whereas biotechnology is a branch of applied science that uses living organisms and their derivatives to produce products and processes. Biocatalysis refers to the chemical process in which the biological catalysts or enzymes speed-up the process of reactions among organic components. Metabolic engineering aims to improve cellular biosynthetic productivity of target metabolites. This is done by redesigning and controlling metabolic pathways, through the utilization of genome-scale modeling, formation of novel pathways, multi-omics analysis and metabolic flux regulation. Biocatalysis and metabolic engineering together seek to create novel products using biological means by using genetic engineering in various microbial chassis with appropriate metabolic repertoires. This book is a valuable compilation of topics, ranging from the basic to the most complex advancements in the field of biotechnology and bioengineering. The advancements in biocatalysis and metabolic engineering have been glanced at in detail. The book will provide comprehensive knowledge to the readers.