The James Webb Space Telescope (JWST), Atacama Large Millimeter Array (ALMA) and a new generation of other ground-based instruments will soon glimpse the first primitive galaxies in the universe, yielding unprecedented insights into the properties of the first stars, the luminosities and spectra of protogalaxies, and the chemical evolution of the early intergalactic medium. In recent years, numerical simulations of the formation of the first stars and galaxies have made significant strides, but considerable work remains to be done to place estimates of the primordial IMF and the morphology, brightness, and colors of primeval galaxies on a firmer footing. The conference was organized around a number of key challenges, with the aim of bringing together leading experts on JWST and ALMA with theorists of early structure formation to forge roadmaps for the next several years.